Helicobacter pylori: comparison of DNA fingerprints provides evidence for intrafamilial infection.

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Helicobacter pylori: comparison of DNA fingerprints provides evidence for intrafamilial infection.

Although a high prevalence of antibodies to Helicobacter pylori has been documented within families, culture and DNA typing of strains from infected children and their parents has not been evaluated. This study aimed to analyse H pylori infection within family groups. Endoscopy, gastric biopsy, and H pylori culture were performed on all eight parents of four children who presented with dyspepsi...

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IHelicobacter pylori: comparison ofDNA fingerprints provides evidence for intrafamilial infection

groups. Endoscopy, gastric biopsy, and H pylori culture were performed on all eight parents of four children who presented with dyspepsia and who had a positive H pylori culture. All biopsy specimens were cultured on Columbia based blood agar under microaerophilic conditions for four days. The DNA from each strain was extracted and electrophoretic patterns were compared after digestion with res...

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Intrafamilial Genotyping of Helicobacter pylori from Faecal DNA

Helicobacter pylori infection, often acquired in early childhood, is a global cause of undernutrition, gastritis, peptic ulcer disease and gastric carcinoma. This study tested the feasibility of using H. pylori shed in the faeces as a source of DNA for non-invasive epidemiological studies. H. pylori DNA was chemically recovered and isolated using a specific biotinylated oligonucleotide probe wi...

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Helicobacter pylori Infection among Patients with Dyspepsia and Intrafamilial Transmission

Introduction Recurrence is an important problem after Helicobacter pylori infection, and intrafamilial transmission has an important role in recurrence. In this study, we aimed to investigate the significance of intrafamilial transmission for recurrence development after treatment as well as its usefulness in prevention. Materials and methods Of the 109 patients who had dyspepsia and underwen...

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Comparison of noninvasive diagnostic tests for Helicobacter pylori infection.

OBJECTIVES Since the (13)C-urea breath test (UBT) has become a highly reliable method for the noninvasive diagnosis of Helicobacter pylori infection, this study was performed in order to compare the sensitivity, specificity and accuracy among noninvasive tests including capsule UBT, conventional UBT and serology in the diagnosis of H. pylori infection. PATIENTS AND METHODS One hundred patient...

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ژورنال

عنوان ژورنال: Gut

سال: 1993

ISSN: 0017-5749

DOI: 10.1136/gut.34.10.1348